Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Tuesday, 12 April 2016

UCA_6B_1: Day 0 – Creation of the column

Rio San Pedro sediment + 1g CaSO4

For the preparation of our column we take a bottle and use the Rio San Pedro mud as the sediment and we added 1 grams of CaSO4 to the bottle. We add some water too to recreate aquatic conditions. We close it and put it in a box so the light couldn’t penetrate through. That will allow the growth of chemosynthetic organisms in the column.
We suppose that in the top of the column aerobic organisms will grow as the concentration of the oxygen is higher, while in the bottom of the column the conditions will be the opposite, so anaerobic organisms will grow.
Another hypothesis is that in the deeper parts of the column the sulphur will be accumulated and microorganisms able to metabolize it will proliferate, however in the surface will be just the opposite as the concentration of the sulphur will be very low.

With this information, we could affirm that the column will be differenced in three zones: the aerobic zone, the microaerophilic zone and the anaerobic zone. The addition of CaSO4 will stimulate the growth of the microorganisms.


  1. All very nice. But... how putting the bottle in the dark help the production of oxygen by anaerobic respiration? :-/

    1. That was a little mistake that we did not notice and we corrected it. Thanks ;-)