Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Sunday, 10 April 2016

UCA_10A_2: Day 28

Rio San Pedro sediment + Paper + 1.5g FeSO4

Hi everyone!

It’s been 2 weeks since the last check and the column has experimented some changes that we will detail below.

First of all, we can see how the black color that started to form in the bottom of the column has expanded all over it, being less consistent as we go up trought the column. That indicates the proliferation of sulphate reducing bacteria. This type of bacteria reduces SO42- ions to S2- ions that bind to Fe2+ in solution generating black coloured FeS.

Our column has been stored in the dark, so no kind of photosintetic organism has been able to grow in it. That means no O2 production in the medium, making it almost anaerobic (still some O2 diffuses into from atmosphere), wich explains the proliferation of the sulphate reducing bacteria (they use SO42 instead of O2 as final electron acceptor)

Besides, we notice the appearance of some strips of orange/brown color near the surface. That indicates the presence of some kind of iron oxidizing bacteria that are generating Fe2O3, due to that little amount of O2 diffusing from atmosphere. This compund provides the color mentioned.

Our dark column

Dark (left) vs light (right) column


  1. Nice. What happened to the light column?

    1. Hi, the light column turned green because of the cyanobacterias. Also we observed red regions.